Characterization of a truncated form of arrestin isolated from bovine rod outer segments
نویسندگان
چکیده
منابع مشابه
Studies on fragments of rod outer segments from bovine retinas.
Monolayers of fragments of our rod segments were formed at an air-water interface. The area of these particles was measured as a function of the pH of the aqueous phase. A maximum area was measured at pH 6.5. The film was characterised by measuring the decrease in surface pressure, after compression, and the half time required to reach constant pressure. At a pH between 6 and 7 the decrease in ...
متن کاملCrystallization of proteins from crude bovine rod outer segments.
Obtaining protein crystals suitable for X-ray diffraction studies comprises the greatest challenge in the determination of protein crystal structures, especially for membrane proteins and protein complexes. Although high purity has been broadly accepted as one of the most significant requirements for protein crystallization, a recent study of the Escherichia coli proteome showed that many prote...
متن کاملPurification of retinol dehydrogenase from bovine retinal rod outer segments.
We purified retinol dehydrogenase from bovine rod outer segments using polyethylene glycol precipitation and hydroxylapatite, concanavalin A-Sepharose CL-4B, and Sepharose CL-6B column chromatography in the presence of NADP. We obtained 13-fold purification of retinol dehydrogenase with specific activity of 61.8 nmol/min/mg and 3.8% recovery. Sodium dodecyl sulfate-polyacrylamide gel electropho...
متن کاملTubulin in bovine retinal rod outer segments.
Bovine rod outer segment (ROS) preparations contain a major 58 kDa protein doublet that was identified by immunoblot as tubulin. Quantification by gel densitometry showed that the total amount of tubulin was 5- to 10-fold higher than that attributable to the rod axoneme, suggesting additional role(s) for tubulin in photoreceptor cells. Approximately 20% of this nonaxonemal tubulin (15% of total...
متن کاملIsolation and characterization of cGMP phosphodiesterase from bovine rod outer segments.
A simple purification method has been developed for isolation of bovine cGMP phosphodiesterase from photoreceptor rod outer segments. The enzyme is peripherally membrane bound in its native state and is present in relatively high concentrations. In the bovine photoreceptors its molar ratio to rhodopsin can be estimated to be as great as 1:40 and not lower than 1: 170. The isolated enzyme is pur...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1994
ISSN: 0961-8368
DOI: 10.1002/pro.5560030215